README for Figure 4E.csv 
*** This file contains the raw data obtained on DNA, DNA-MJ1647, DNA-MJ1647 deltaTM and DNA-A3 complexes using bridging assay experiments represented in Figure 3A of 
Article: DNA-bridging by an archaeal histone variant via a unique tetramerisation interface 
Authors: Ofer, Blombach, Erkelens, Barker, Schwab, Smollett, Matelska, Fouqueau, van der Vis, Kent, Pinotsis, Dame and Werner
Journal:  
DOI:
Corresponding author: rtdame@chem.leidenuniv.nl and f.werner@ucl.ac.uk

Legend Figure 4:MJ1647 tetramers compact and bridge DNA strands. 
(A-C) Tethered Particle Motion (TPM) experiments testing DNA compaction by A3 (G), MJ1647 (H) and MJ1647?C (I). Root Mean Square displacement (RMS) values are plotted as a function of histone concentration. The RMS values were obtained from fitting the data with a Gaussian distribution. Error bars represent the propagated standard deviation from at least two individual measurements. Dashed line is a line to guide the eye. For MJ1647, the roman numbers (I, II and III) represent three populations at ~150, 132 and 112 nm at 36 nM MJ1647 used for end-to-end distance calculations (see panel D).
(D) Histograms of calculated end-to-end distances of the three populations observed at 36 nM MJ1647. Of each population, the 25 beads closed to the fitted RMS value were selected and the end-to-end distance was calculated of the 2.5% most distant positions with respect to the center of the beads. Histograms were fit with a skewed normal distribution. Insert: pairwise distribution plot of the differences between the end-to-end distance peaks I, II and III. Histograms were fitted with a Gaussian distribution.
(E) DNA bridging assays. DNA recovery (%) is plotted as a function of protein concentration. Data are plotted as mean values of three independent measurements and error bars represent the standard deviation. Dashed line is a line to guide the eye.

*** The data were obtained using bridging assay experiments as described in the associated article. 

***The data labeled overview represents the values plotted in the graph of figure 4E. The raw data contains the individual replicates.
Column A: Sample number
Column B: Presence of bait DNA in sample
Column C: Presence of 32P-labeled prey DNA in sample
Column D: Amount of MJ1647/MJ1647 deltaTM/A3 added to sample in micromolar
Column E: Counts per minutes 
Column F: DNA recovery (%)

Columns B, C and D indicate the contents of the sample. Column E indicates the radioactivity of the sample. 
Column F indicates the DNA recovery, which is calculated by subtracting the value in column E of the control 
(sample minus bait DNA) from the sample itself. This value is then divided by the values in column E of sample 1 
and multiplied by 100%. 









